Your browser doesn't support javascript.
loading
Establishment of culture system of Silybum marianum hairy roots and determination of silybin / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2005-2010, 2014.
Artigo em Chinês | WPRIM | ID: wpr-299842
ABSTRACT
This research uses six Agrobacterium rhizogenes R1601, R15384, R1000, A4, R1025 and R1 to infect silymarin explants to induce hairy roots and silibin. All of the six A. rhizogenes can induce Silybum marianum to generate hairy roots and the A. rhizogene A4 shows comparatively high infection on the plant. This research determines the condition to induce silymarin hairy roots by the factors of infection time, pre-culturing, co-culturing and pH value. The fact that MS liquid medium fits the proliferation of silymarin hairy roots is determined. Through PCR molecular identification, it can be seen that the DNA plasmids in the A. rhizogenes are successfully integrated into the genome of transformed roots. Using liquid chromatography, it is determined that the silibin content in silymarin hairy roots is 2.5 times that in the plant In this research, the silymarin hairy roots culturing system is established, which lays a foundation for the study of culturing silymarin hairy roots and producing silibin.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Silimarina / Transformação Genética / Química / Raízes de Plantas / Técnicas de Cultura de Células / Silybum marianum / Agrobacterium / Genética / Métodos Tipo de estudo: Estudo prognóstico Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2014 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Silimarina / Transformação Genética / Química / Raízes de Plantas / Técnicas de Cultura de Células / Silybum marianum / Agrobacterium / Genética / Métodos Tipo de estudo: Estudo prognóstico Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2014 Tipo de documento: Artigo