Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 445-448, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-300011
ABSTRACT
<p><b>OBJECTIVES</b>To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.</p><p><b>METHODS</b>The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.</p><p><b>RESULTS</b>PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.</p><p><b>CONCLUSION</b>Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Terapia Genética
/
Reação em Cadeia da Polimerase
/
Fator de Crescimento Transformador beta
/
Proteínas Morfogenéticas Ósseas
/
Engenharia Tecidual
/
Proteína Morfogenética Óssea 7
/
Células-Tronco Mesenquimais
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2003
Tipo de documento:
Artigo
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