The effect of ultra high molecular weight polyethylene particles on macrophage / 中华外科杂志
Chinese Journal of Surgery
;
(12): 852-855, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-300597
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of ultra high molecular weight polyethylene (UHMWPE) particles on macrophages and evaluate the expression of NFAT2, a key transcriptional factor for osteoclast differentiation.</p><p><b>METHODS</b>From November 2004 to February 2005, macrophages were co-cultured with UHMWPE particles. When observed at different times, the proliferation activity of macrophages was analyzed by MTT and the expression of calcineurin (CaN) and NFAT2 by immunohistochemical and RT-PCR method respectively.</p><p><b>RESULTS</b>The macrophages phagocytosed UHMWPE particles in an early time, the expression of CaN and NFAT2 was increased, while the proliferation activity was not enhanced.</p><p><b>CONCLUSIONS</b>UHMWPE particles can stimulate macrophages to phagocytose significantly, and enhance the expression of the transcriptional factor NFAT2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fagocitose
/
Farmacologia
/
Fisiologia
/
Polietilenos
/
Técnicas In Vitro
/
RNA Mensageiro
/
Fatores de Transcrição NFATC
/
Genética
/
Prótese Articular
/
Macrófagos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS