LC/MS guided approach to discovering nephroprotective substances from Huangkui capsule / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 66-73, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-300822
ABSTRACT
To discover the nephroprotective substances from Huangkui capsule.The components of Huangkui capsule were isolated by preparative liquid chromatography, and the active components were screened by LC/MS and identified. The adriamycine-injured HK-2 cells were treated with various active components with different concentrations, and the malonaldehyde (MDA) content, adenosine triphosphate (ATP) level and mitochondrial oxygen consumption rate were measured to verify the protective activity of the compounds.Four active components in Huangkui capsule were identified to exert nephroprotective effects. Fifteen flavanoids from these four components were tentatively identified by LC/MS, and hyperin, myricetin, quercetin, rutin and isoquercetin were confirmed. Hyperin, myricetin quercetin and rutin showed dose-dependent protective effects on injured HK-2 cells. Espacially, hyperin significantly reduced MDA content, quercetin and rutin significantly increased ATP level, and myricetin significantly increased mitochondrial oxygen consumption rate.Hyperin, myricetin, querctein and rutin might be the potential nephroprotective compounds in Huangkui capsule, their effects may be related to the inhibition of lipid peroxidation and the alleviation of mitochondrial damage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Consumo de Oxigênio
/
Farmacologia
/
Quercetina
/
Rutina
/
Espectrometria de Massas
/
Flavonoides
/
Linhagem Celular Transformada
/
Medicamentos de Ervas Chinesas
/
Peroxidação de Lipídeos
/
Doxorrubicina
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2017
Tipo de documento:
Artigo
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