Effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor cells MC3T3-E1 / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 575-580, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-300845
ABSTRACT
To investigate the effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor (MC3T3-E1) cells.miR-705 mimics, inhibitors and negative control were transfected into MC3T3-E1 cells. Alkaline phosphates (ALP) staining were performed and quantified after 7 days of osteogenic medium induction. The mRNA and protein expression levels of runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) were detected by real-time RT-PCR and Western blot after 14 days of osteogenic induction. Alizarin red staining was performed and quantified in MC3T3-E1 cells after 21 days of osteogenic induction.After 7 days of osteogenic induction, ALP staining showed that overexpression of miR-705 significantly reduced ALP activity, whereas knockdown of miR-705 increased ALP activity (all<0.05). Consistently, after 14 days of osteogenic induction, mRNA and protein expressions of Runx2 and OCN were suppressed by overexpression of miR-705, whereas they were promoted by knockdown of miR-705 (all<0.05). After 21 days of osteogenic induction, alizarin red staining showed that overexpression of miR-705 significantly reduced the formation of mineralized node, the opposite results were found in miR-705 knockdown group (all<0.05).miR-705 can inhibit the osteogenic differentiation of MC3T3-E1 cells.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteogênese
/
Farmacologia
/
Calcificação Fisiológica
/
Regulação para Baixo
/
Osteocalcina
/
Diferenciação Celular
/
MicroRNAs
/
Fosfatase Alcalina
/
Subunidade alfa 1 de Fator de Ligação ao Core
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2016
Tipo de documento:
Artigo
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