Evaluation of deoxyribonuclease activity in seminal plasma of ejaculated chicken semen / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 213-216, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-300889
ABSTRACT
<p><b>AIM</b>To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen and the stability of DNA was examined by adding lipofection reagents.</p><p><b>METHODS</b>A PCR fragment (249 bp) of pEGFPN-1 vector was used as the DNA substrate and was incubated with the seminal plasma at 40 degree C for 30 min. Then, the whole reaction solution was subjected to agarose gel electrophoresis and the DNA size was evaluated under UV light.</p><p><b>RESULTS</b>The DNA substrate was completely diminished after incubation with seminal plasma. However, the substrate was intact after incubation with heat-treated seminal plasma or incubation with seminal plasma in the presence of 0.5 mmol/L approximately 5 mmol/L EDTA. The substrate was stabilized in the seminal plasma by the addition of commercially available lipofection reagents.</p><p><b>CONCLUSION</b>The DNase activity is present in the seminal plasma of ejaculated chicken semen. However, DNA is stable in the liposomal-DNA complex.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Sêmen
/
DNA
/
Cátions Bivalentes
/
Galinhas
/
Ácido Edético
/
Primers do DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Desoxirribonucleases
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2003
Tipo de documento:
Artigo
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