Changes of Src-suppressed C kinase substrate expression in cytokine induced reactive C6 glioma cells / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 101-106, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-300992
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effect of tumor necrosis factor-alpha (TNF-alpha) on the Src-suppressed C kinase substrate (SSeCKS) in C6 glioma cells.</p><p><b>METHODS</b>Cultured C6 glioma cells were randomly divided into two groups. In time-dependent group, cells were cultured with TNF-alpha (2 ng/mL) for 0 h, 1 h, 3 h, 6 h, 12 or 24 h, respectively; in dose-dependent group, cells were cultured with TNF-alpha (0 ng/mL, 0.02 ng/mL, 0.2 ng/mL, or 2 ng/mL) for 6 h. The expression of SSeCKS was detected by Realtime PCR and Western blot analysis, and immunocytochemistry was used to investigate SSeCKS's subcellular localization.</p><p><b>RESULTS</b>TNF-alpha induced rapid phosphorylations of protein kinase C (PKC) substrates in C6 glioma cells, and upregulated SSeCKS expression in a time and concentration dependent manner. Immunocytochemistry suggested that SSeCKS was localized in the cyroplasm and the leading end of podosomal extensions in control groups, while TNF-alpha induced translocation of SSeCKS perinuclear. This effect could be partly reversed by PKC inhibitor Ro-31-8220.</p><p><b>CONCLUSION</b>TNF-alpha activates PKC and upregulates SSeCKS expression in C6 glioma cells. These effects are associated with PKC activity, suggesting that SSeCKS plays a role in response to glia activation in PKC mediated pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Neoplasias Encefálicas
/
Proteína Quinase C
/
Imuno-Histoquímica
/
Células Tumorais Cultivadas
/
Transdução de Sinais
/
Distribuição Aleatória
/
Regulação da Expressão Gênica
/
Astrócitos
/
Fator de Necrose Tumoral alfa
Limite:
Animais
Idioma:
Inglês
Revista:
Neuroscience Bulletin
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS