Absorption of recombinant hirudin in rats GI tract / 药学学报

ABSTRACT

<p><b>AIM</b>To investigate the absorption and distribution of recombinant hirudin-2 (rHV2) in the GI tract in rats after oral administration.</p><p><b>METHODS</b>Using HPLC, fluorescence spectrophotometry and confocal laser scanning microscopy to measure the amount of intact rHV2 absorbed into gastrointestinal mucosa and blood.</p><p><b>RESULTS</b>HPLC spectrum showed that there were intact rHV2 molecules in plasma after 1 h of oral administration. After oral administration for 3 h, 1.2%-26.8% of fluorescence was found in the GI tract in rats. Chromatographic analysis showed that 2.27%-38.75% of fluorescence recovered from the GI tract luminal contents and mucosa was eluted at the void volume of a Sephadex G-25 column. Microscopic examination showed that FITC-rHV2 was taken up throughout the whole small intestine but the ileum appeared to be a preferred site for FITC-rHV2 transport in rats.</p><p><b>CONCLUSION</b>rHV2 may partially survive in the GI lumen and subsequently absorbed in active form by gastrointestinal tract.</p>