Monitoring sub-nanogram amount of acetylspiramycin in human urine using flow injection analysis with chemiluminescence detection / 药学学报
Acta Pharmaceutica Sinica
;
(12): 64-67, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-301147
ABSTRACT
<p><b>AIM</b>To establish a new and simple flow injection method for the rapid determination of acetylspiramycin (ASPM).</p><p><b>METHODS</b>ASPM was determined by chemiluminescence (CL) method combined with flow injection (FI) technology, which was based on the inhibitive effect of ASPM on the chemiluminescence reaction of the luminol-K3Fe (CN)6 system.</p><p><b>RESULTS</b>The decrease of chemiluminescence intensity was proportional to the logarithm of ASPM concentration (0.1-100) microgram.L-1, the detection limit was 40 ng.L-1 (3 sigma). The whole process, including sampling and washing, could be completed in 0.5 min with a RSD less than 3.0% (n = 5).</p><p><b>CONCLUSION</b>The FI-CL method is of both high sensitivity and good selectivity giving a throughput of 120 h-1. The proposed method was applied successfully to the determination of ASPM in pharmaceutical preparations and human urine without any pre-treatment. It was found that the ASPM concentration reached its maximum after being orally administrated for two hours.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Urina
/
Sangue
/
Espiramicina
/
Química
/
Análise de Injeção de Fluxo
/
Medições Luminescentes
/
Luminol
/
Microquímica
/
Antibacterianos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
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