Simultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detection / 药学学报
Acta Pharmaceutica Sinica
;
(12): 56-59, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-301149
ABSTRACT
<p><b>AIM</b>To establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine A, imperialine-3 beta-glucoside and yibeinoside A.</p><p><b>METHODS</b>A Nova-Pak C18 column (150 mm x 3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL.min-1.</p><p><b>RESULTS</b>The linear range of peimissine was 13.1-288.2 mg.L-1 (r2 = 0.9975), imperialine-3 beta-glucoside 7.7-169.4 mg.L-1 (r2 = 0.9993), yibeinoside A 7.3-160.6 mg.L-1 (r2 = 0.9997), imperialine 16.5-363.0 mg.L-1 (r2 = 0.9992), sinpeinine A 8.7-191.4 mg.L-1 (r2 = 0.9942).</p><p><b>CONCLUSION</b>The method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Medicamentos de Ervas Chinesas
/
Cevanas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Fritillaria
/
Alcaloides
/
Glucosídeos
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS