Determination of daurisoline in rabbit plasma and study on its pharmacokinetics / 药学学报

ABSTRACT

<p><b>AIM</b>To establish an HPLC method for the determination of daurisoline (DS) in rabbit plasma and investigate its pharmacokinetic characteristics after intravenous administration.</p><p><b>METHODS</b>Dauricine was used as internal standard. The plasma samples were deproteinated with acetonitrile and extracted with two-step dichloromethane. Acetonitrile-water-triethylamine (18:82:0.28, pH 3) was used as mobile phase at a flow rate of 1.0 mL.min-1. The UV-detector was set at 284 nm.</p><p><b>RESULTS</b>The linear range was 0.05-20.00 micrograms.h.mL-1 with correlation coefficients 0.9996. The limit of quantitation (LOQ) was 0.05 mg.L-1 of plasma. The absolute and relative recoveries were above 80% and (101 +/- 5)%, respectively. The intra- and inter-assay coefficient of variation were 1.9%-5.6% and 3.5%-6.5%, respectively. The plasma concentration-time profiles were adequately described by a two-compartment open model.</p><p><b>CONCLUSION</b>A sensitive, precise and accurate method for the determination of DS in plasma was described, which can be used in its pharmacokinetic studies.</p>