The correlation of synuclein-γ and matrix metalloproteinase 9 in breast cancer / 中华外科杂志
Chinese Journal of Surgery
;
(12): 641-644, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-301217
ABSTRACT
<p><b>OBJECTIVES</b>To evaluate the expression of synuclein-γ (SNCG) and metalloproteinase 9 (MMP-9) both in the invasive ductal breast cancer samples and T47D and T47D(SNCG)- cell lines, to investigate the correlation between SNCG and MMP-9.</p><p><b>METHODS</b>Totally 96 invasive ductal breast cancer samples (female, mean age of (56 ± 8) years) were collected between June 2009 and June 2012. The expressions of SNCG and MMP-9 were investigated by immunohistochemistry. T47D and SNCG knock down T47D(SNCG)- cell lines were established and SNCG and MMP-9 protein expression were investigated by Western blot and gene expression by real-time PCR.</p><p><b>RESULTS</b>Among 96 samples, 26 (27.1%) of them co-expressed SNCG and MMP-9, 30(31.2%) of them expressed neither SNCG nor MMP-9. The expression of SNCG was correlated with the expression of MMP-9 (r = 0.655, P = 0.000).SNCG mRNA level of T47D cell line was 13.5 fold of T47D(SNCG)- cell line and SNCG protein expression was 2.1 fold. While MMP-9 mRNA level of T47D cell line was 7.3 fold of T47D(SNCG)- cell line and MMP-9 protien expression was 1.6 fold.When SNCG was knocked down, the expression of MMP-9 decreased.</p><p><b>CONCLUSIONS</b>SNCG and MMP-9 are significantly correlated with each other in breast cancer. SNCG may promote the invasion and metastasis of breast cancer mediated by up-regulating the expression of MMP-9.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias da Mama
/
RNA Mensageiro
/
Expressão Gênica
/
Metaloproteinase 9 da Matriz
/
Gama-Sinucleína
/
Reação em Cadeia da Polimerase em Tempo Real
/
Genética
/
Metabolismo
/
Proteínas de Neoplasias
Limite:
Idoso
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2013
Tipo de documento:
Artigo
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