Effect of GBE50 on delayed rectifier potassium current of ventricular myocytes in ischemic guinea pig / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 444-448, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-301541
ABSTRACT
<p><b>OBJECTIVE</b>Ginkgo biloba extract 50 (GBE50) is a new multicomponent drug with a polyvalent action extracted from the leave of Ginkgo biloba. The aim of this experiment was to study the effects of GBE50 on delayed rectifier potassium current (I(K)) in ventricular myocytes under normal and simulated ischemia conditions in guinea pigs.</p><p><b>METHODS</b>Single ventricular myocytes were isolated by an enzymatic dissociation method. I(K) were recorded by whole-cell patch clamp technique in voltage clamp mode. GBE50 was added to the perfusion chamber from low to high concentrations (25, 50,100 mg/L) in normal condition. Different concentrations of GBE50 (25, 50, 100 mg/L) were prepared with simulated ischemic fluid.</p><p><b>RESULTS</b>(1) Under normal condition, 100 mg/L GBE50 decreased I(K) (n = 7, P < 0.05). (2) Under ischemia condition, it was observed that I(K) was inhibited (n = 8, P < 0.05). (3) Perfusion with ischemia solution containing 50 mg/L (n = 8, P > 0.05) and 100 mg/L GBE50 (n = 6, P > 0.05) could reverse the decrease of I(K).</p><p><b>CONCLUSION</b>GBE50 significantly decreased I(K) in a concentration-dependent manner. GBE50 could alleviate the electrophysiological heterogeneity of myocardium to prevent ischemic myocardium from arrhythmia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Extratos Vegetais
/
Células Cultivadas
/
Isquemia Miocárdica
/
Técnicas de Patch-Clamp
/
Ginkgo biloba
/
Miócitos Cardíacos
/
Canais de Potássio de Retificação Tardia
/
Cobaias
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2010
Tipo de documento:
Artigo
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