Rapid inhibitory effect of glucocorticoids on peak of Ca2+i and PLC in airway smooth muscle / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 440-443, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-301542
ABSTRACT
<p><b>OBJECTIVE</b>In this study, we pretreated the mice ASMCs by dexamethasone (Dex) within 10 min, to test the peak of [Ca2+]i and phospho-PLCbeta (ser1105) in the cells by treated with Ach.</p><p><b>METHODS</b>The peak of [Ca2+]i was measured by Fura-2/AM methods and the phospho-PLCbeta-ser1105 was by Western blot, and compared with dexamethasone pretreated groups. Glucocorticoid receptor antagonist RU486 and the protein synthesis inhibitor cycloheximide groups were settled in our study.</p><p><b>RESULTS</b>Glucocorticoids (GCs) significantly decreased the resting values and peak of [Ca2+]i elevation and elevated the intracellular levels of phospho-PLCbeta (ser1105) in 10 min. Neither the RU486 nor cycloheximide could alter the inhibitory effects of glucocorticoids stated above.</p><p><b>CONCLUSION</b>Our results demonstrate that glucocorticoids exert rapid inhibitory effects. The series of signal changes in this process that restrain the peak of [Ca2+]i may be responsible for the rapid nongenomic inhibitory effects of GCs by reducing the activity of PLC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Traqueia
/
Dexametasona
/
Mifepristona
/
Células Cultivadas
/
Cálcio
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Fosfolipase C beta
/
Glucocorticoides
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2010
Tipo de documento:
Artigo
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