Preparation of clenbuterol monoclonal antibody with subtractive immunization method / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 573-576, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-301558
ABSTRACT
<p><b>AIM</b>To obtain Clenbuterol monoclonal antibodies.</p><p><b>METHODS</b>Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.</p><p><b>RESULTS</b>The mice obtained tolerance to BSA by subtractive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific clenbuterol monoclonal antibody was obtained at last.</p><p><b>CONCLUSION</b>Monoclonal antibodies to micromolecule contaminant be prepared by subtractive immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Soroalbumina Bovina
/
Imunização
/
Clembuterol
/
Alergia e Imunologia
/
Hibridomas
/
Metabolismo
/
Métodos
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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