Sensitivity of interferon-gamma release assay T-SPOT. TB in diagnosing extrapulmonary tuberculosis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 449-452, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-301674
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the sensitivity of an interferon-gamma release assay T-SPOT. TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis.</p><p><b>METHOD</b>Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT. TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells (PBMCs).</p><p><b>RESULTS</b>T-SPOT. TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5% (95% CI 84.8% - 100%). The median of spot forming cells (SFCs) in response to early secreting antigen target 6 peptides was 196/10(6) PBMCs (interquartile range, 72-532/10(6) PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10(6) PBMCs (interquartile range, 72-568/10(6) PBMCs), and the median of the incorporate SFCs was 612/10(6) PBMCs (interquartile range, 192-1 152/10(6) PBMCs).</p><p><b>CONCLUSION</b>T-SPOT. TB is highly sensitive in diagnosing extrapulmonary tuberculosis.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tuberculose
/
Leucócitos Mononucleares
/
Sensibilidade e Especificidade
/
Interferon gama
/
Diagnóstico
/
Testes de Liberação de Interferon-gama
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2009
Tipo de documento:
Artigo
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