Screening for inhibitor of vascular endothelial growth factor from random peptide library / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 540-543, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-301941
ABSTRACT
<p><b>OBJECTIVE</b>To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.</p><p><b>METHODS</b>Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.</p><p><b>RESULTS</b>Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.</p><p><b>CONCLUSION</b>The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Farmacologia
/
Sítios de Ligação
/
Ensaio de Imunoadsorção Enzimática
/
Fatores de Crescimento Endotelial
/
Linfocinas
/
Biblioteca de Peptídeos
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Fatores de Crescimento do Endotélio Vascular
/
Fator A de Crescimento do Endotélio Vascular
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2002
Tipo de documento:
Artigo
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