Synergistic effect of toremifene and cisplatin on human lung cancer cell line A549 / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 537-539, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-301942
ABSTRACT
<p><b>OBJECTIVE</b>To study the toxic effect of toremifene (TOR) and its synergistic effect with cisplatin (DDP) on human lung adenocarcinoma cell line A549.</p><p><b>METHODS</b>The cytotoxic effects of these agents on human lung cancer cell line A549 were monitored by a tetrazolium-based colorimetric assay (MTT assay). The cell cycle and DNA content were detected by flow cytometer technic. p21 expression level was monitored by Western blot.</p><p><b>RESULTS</b>Toremifene inhibited the growth of A549 cell, with > or = 5 micromol/L significantly enhancing the chemosensitivity of cisplatin. TOR enhanced the antitumor activity of DDP at S, G(2) and M phases of cells. And p21 expression was increased after TOR and DDP had been given.</p><p><b>CONCLUSION</b>Toremifene (> or = 5 micromol/L) combined with cisplatin shows significant synergistic anti-tumor effect on A549 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Ensaios de Seleção de Medicamentos Antitumorais
/
Células Tumorais Cultivadas
/
Protocolos de Quimioterapia Combinada Antineoplásica
/
Divisão Celular
/
Cisplatino
/
Toremifeno
/
Resistencia a Medicamentos Antineoplásicos
/
Sinergismo Farmacológico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2002
Tipo de documento:
Artigo
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