Changes of sarcoplamic reticular Ca(2+)-ATPase and IP(3)-I receptor mRNA expression in patients with atrial fibrillation / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 664-667, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-302231
ABSTRACT
<p><b>OBJECTIVE</b>To investigate changes in the expression of sarcoplamic reticular Ca(2+)-ATPase (SERCA) and IP(3)-I receptors (IP(3)R(1)) mRNA in patients with atrial fibrillation.</p><p><b>METHODS</b>Thirty-eight patients with mitral stenosis undergoing open heart surgery were studied. 100 mg of atrial tissue was obtained during surgery from the right appendage and the right atrium. The amount of messenger ribonucleic acid (mRNA) amount of SERCA and IP(3)R(1) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and normalized to the mRNA levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH).</p><p><b>RESULTS</b>Levels of mRNA expression of SERCA in patients with AF, as compared with subjects in sinus rhythm, was lower and that of IP(3)R(1) was higher. The longer AF was sustained, the higher the levels of mRNA. There was no significant difference between right atrial free wall and right appendage.</p><p><b>CONCLUSIONS</b>The expression changes of SERCA and IP3R mRNA may correlate with the initiation or maintenance of AF.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fibrilação Atrial
/
RNA Mensageiro
/
Canais de Cálcio
/
Expressão Gênica
/
ATPases Transportadoras de Cálcio
/
Receptores Citoplasmáticos e Nucleares
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático
/
Receptores de Inositol 1,4,5-Trifosfato
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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