T cell receptor Vbeta gene bias in rheumatoid arthritis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 856-859, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-302288
ABSTRACT
<p><b>OBJECTIVE</b>To explore the pathogenesis of rheumatoid arthritis (RA) by studying the expression of T cell receptors (TCRs).</p><p><b>METHODS</b>T cell receptor Vbeta (TCR Vbeta) gene usage and expression were analyzed from synovial membrane and peripheral blood of 8 RA patients, 2 osteoarthritis patients and 2 accident amputees. The complementary determining region 3 (CDR3) of 25 TCR Vbeta subfamily genes in unselected T cell populations were amplified semi-quantitatively by reverse transcription-polymerase chain reaction (RT-PCR). The products were further studied by genescan for frequency of Vbeta usage.</p><p><b>RESULTS</b>The numbers of Vbeta subfamilies expressed by T cells from RA peripheral blood and synovial membrane were not significantly restricted. More importantly, biased Vbeta gene expression in RA synovium was observed and Vbeta6, Vbeta17, and Vbeta22 genes were the predominant subfamilies. It was noteworthy that the expression of Vbeta17 in RA synovium was significantly increased.</p><p><b>CONCLUSION</b>Our data were consistent with the hypothesis that several antigen or superantigen-driven processes may be involved in the pathogenesis of RA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artrite Reumatoide
/
Membrana Sinovial
/
Linfócitos T
/
Genes Codificadores da Cadeia beta de Receptores de Linfócitos T
/
Alergia e Imunologia
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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