Development of a rat C6 brain tumor model / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume.</p><p><b>METHODS</b>C6 cell suspension (10 microl) containing 10 g/L agarose and 1 x 10(6) cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains.</p><p><b>RESULTS</b>Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%.</p><p><b>CONCLUSION</b>A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.</p>