Inhibitory effect of genistein on the proliferation of Raji cells and its related mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 971-975, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-302364
ABSTRACT
This study was aimed to investigate the anti-proliferative effect of genistein (Gen) on BCL-6 positive Raji cells and its related mechanism. Trypan blue staining and MTT method were used to analyze the anti-proliferative effect of Gen on Raji cells. Cell apoptosis, protein expression and the interaction of BCL-6 and NCoR were detected by PI/AV dual staining, Western blot and Co-IP method, respectively. The results showed that Gen had time- and dose-dependent inhibitory effect on Raji cell proliferation and induced apoptosis. Different dose of Gen had no significant effect on the expression of BCL-6 and NCoR, but could inhibit the binding of BCL-6 and NCoR. It is concluded that Gen shows inhibitory effect on BCL-6 positive lymphoma cells, which can be as a adjuvant therapy for combined rituximab with chemotherapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Linfócitos
/
Apoptose
/
Genisteína
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Relação Dose-Resposta a Droga
/
Proteínas Proto-Oncogênicas c-bcl-6
/
Correpressor 1 de Receptor Nuclear
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2014
Tipo de documento:
Artigo
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