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Effects of rapamycin on biological characteristics of bone marrow mesenchymal stem cells from patients with aplastic anemia / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 762-766, 2014.
Artigo em Chinês | WPRIM | ID: wpr-302403
ABSTRACT
This study was aimed to investigate the effects of rapamycin on biological function and autophagy of bone marrow mesenchymal stem cells (BM-MSC) from patients with aplastic anemia so as to provide experimental basis for the clinical treatment of aplastic anemia (AA) with rapamycin. BM-MSC were treated with different concentrations of rapamycin (0, 10, 50, 100 nmol/L) for 48 h, the expression of LC3B protein was detected by Western blot to observe the effect of rapamycin on cell autophagy; cell apoptosis and cell cycles were detected by flow cytometry; the proliferation of BM-MSC of AA patients was measured by cell counting kit-8; the adipogenic differentiation of BM-MSC were tested by oil red O staining after adipogenic induction for 2 weeks; the adipogenic related genes (LPL, CFD, PPARγ) were detected by real-time PCR. The results showed that the proliferation and adipogenesis of BM-MSC of AA patients were inhibited by rapamycin. Moreover, the autophagy and apoptosis of BM-MSC were increased by rapamycin in a dose-dependent way.Rapamycin arrested the BM-MSC in G0/G1 phase and prevented them into S phase (P < 0.05). It is concluded that rapamycin plays an critical role in inhibiting cell proliferation, cell cycles, and adipogenesis, these effects may be related with the autophagy activation and mTOR inhibition resulting from rapamycin.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Autofagia / Células da Medula Óssea / Transdução de Sinais / Ciclo Celular / Células Cultivadas / Apoptose / Sirolimo / Biologia Celular / Proliferação de Células Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Autofagia / Células da Medula Óssea / Transdução de Sinais / Ciclo Celular / Células Cultivadas / Apoptose / Sirolimo / Biologia Celular / Proliferação de Células Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2014 Tipo de documento: Artigo