Human cytomegalovirus UL138 open reading frame is highly conserved in clinical strains / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 107-111, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-302639
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains.</p><p><b>METHODS</b>HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains.</p><p><b>RESULTS</b>UL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities of UL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleotide mutations were substitutions. The spatial structure and post-translational modification sites of UL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV UL138 sequence variations were not definitely related with different clinical symptoms.</p><p><b>CONCLUSION</b>HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Proteínas Virais
/
Dados de Sequência Molecular
/
Química
/
Fases de Leitura Aberta
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Classificação
/
Estrutura Secundária de Proteína
/
Infecções por Citomegalovirus
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2009
Tipo de documento:
Artigo
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