Retinoic acid down-regulates bone morphogenetic protein 7 expression in rat with cleft palate / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 28-31, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-302703
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of retinoic acid (RA) on expression of bone morphogenetic protein 7 (BMP-7) in rat fetus with cleft palate, and the effects of RA on proliferation and apoptosis of osteoblasts.</p><p><b>METHODS</b>All-trans RA (ATRA) was used to induce congenital cleft palate in Wistar rat BMP-7 mRNA expression in maxillary bone tissue of fetal rats was measured by Northern blotting analysis. Flow cytometry and MTT assay were used to measure the apoptosis and proliferation of ATRA-treated MC-3T3-E1 cells. BMP-7 mRNA and protein expressions in ATRA-treated MC-3T3-E1 cells were detected by RT-PCR and Western blotting analysis.</p><p><b>RESULTS</b>ATRA could induce cleft palate of rat fetus. The incidence rate of cleft palate induced by 100 mg/kg AT-RA (45.5%) was significantly higher than 50 mg/kg ATRA (12.5%, P < 0.05). BMP-7 mRNA expression decreased in maxillary bone tissue of rat fetus with cleft palate. MC-3T3-E1 cells proliferation treated with 1 x 10(-6) mol/L ATRA decreased by 60%, the cell apoptosis increased by 2 times. BMP-7 mRNA and protein levels in MC-3T3-E1 cells treated with 1 x 10(-6) mol/L ATRA decreased by 60% and 80%, respectively, compared with ATRA-untreated cells (P < 0.05).</p><p><b>CONCLUSIONS</b>BMP-7 may play an important role in embryonic palate development RA may possess the ability to down-regulate cell proliferation through regulation of BMP-7 gene expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Tretinoína
/
Sequência de Bases
/
Regulação para Baixo
/
Northern Blotting
/
Fissura Palatina
/
Células 3T3
/
Ratos Wistar
/
Apoptose
/
Primers do DNA
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2008
Tipo de documento:
Artigo
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