Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 577-580, 2004.
Article
em Zh
| WPRIM
| ID: wpr-302759
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.</p><p><b>METHODS</b>Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.</p><p><b>RESULTS</b>SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.</p><p><b>CONCLUSION</b>SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Células Cultivadas
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Fator de Crescimento Transformador beta
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Colágeno
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Estresse Oxidativo
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Ácidos Cumáricos
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Hepatócitos
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Biologia Celular
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Proliferação de Células
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Células Epiteliais
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Article