Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells / 药学学报

ABSTRACT

<p><b>AIM</b>To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.</p><p><b>METHODS</b>Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.</p><p><b>RESULTS</b>SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.</p><p><b>CONCLUSION</b>SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.</p>