Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes / 药学学报
Acta Pharmaceutica Sinica
; (12): 509-513, 2004.
Article
em En
| WPRIM
| ID: wpr-302775
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na+/H+ exchanger inhibitor--amiloride.</p><p><b>METHODS</b>Single ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques.</p><p><b>RESULTS</b>Exposure to amiloride (10 -100 micromol x L(-1)), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak (Ca,L), But amiloride did not change the shape of their I - V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 micromol x L(-1) amiloride, I(Kr) was slightly depressed and I(Ks) did not change. Amiloride (1 - 100 micromol x L(-10) depressed I(K1) in a concentration-dependent manner.</p><p><b>CONCLUSION</b>Amiloride depressed potassium and calcium currents, which may give support to its uses in some diseases of the cardiovascular system.</p>
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Assunto principal:
Farmacologia
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Separação Celular
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Trocadores de Sódio-Hidrogênio
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Técnicas de Patch-Clamp
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Canais de Cálcio Tipo L
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Canais de Cálcio Tipo T
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Biologia Celular
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Canais de Potássio Corretores do Fluxo de Internalização
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Canais de Potássio de Abertura Dependente da Tensão da Membrana
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Miócitos Cardíacos
Limite:
Animals
Idioma:
En
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Article