Expression of VEGF in endothelial cells and the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside / 药学学报
Acta Pharmaceutica Sinica
;
(12): 406-409, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-302798
ABSTRACT
<p><b>AIM</b>To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304) and the inhibitory effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (ST I) in vitro.</p><p><b>METHODS</b>Exposure to 2.5 mg x L(-1) LPC or LPC + ST I for 24 hours, VEGF protein was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, VEGF mRNA expression in ECV304 was examined by in situ hybridization. VEGF165 mRNA was examined by RT-PCR and Realtime RT-PCR.</p><p><b>RESULTS</b>LPC upregulated VEGF protein and VEGF mRNA expression in the ECV304 cells. ST I was shown to markedly inhibit the LPC-induced increase of VEGF protein and VEGF165 mRNA (P < 0.001).</p><p><b>CONCLUSION</b>LPC can induce a strong expression of VEGF in ECV304 cells and ST I can inhibit it.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Estilbenos
/
Veias Umbilicais
/
RNA Mensageiro
/
Lisofosfatidilcolinas
/
Células Cultivadas
/
Química
/
Polygonum
/
Biologia Celular
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
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