Your browser doesn't support javascript.
loading
Activity and quality comparison of the engineered protein Staphylococcus aureus alpha-hemolysin purified with gel filtration chromatography and Ni-NTA / 生物工程学报
Chinese Journal of Biotechnology ; (12): 176-180, 2009.
Artigo em Chinês | WPRIM | ID: wpr-302839
ABSTRACT
The alpha-hemolysin protein of Staphylococcus aureus, which was expressed in Escherichia coli BL21 (DE3) with recombinant pET32a(+)-alpha-HL plasmid, was purified with gel filtration chromatography (GFC) and Ni-NTA spin columns. The quality and biological characteristic were compared. First, the purified products were analyzed with SDS-PAGE, and the expected protein band was with a molecular mass of 53 kD. Second, protein concentration was determined by the method of Bradford, and the median hemolytic dose potency (HD50) was finally analyzed with rabbit erythrocyte. The protein purified with GFC was 0.337 mg/mL, its hemolysis activity was 1519 HU/mg, and hemolysin yield was 14.04%. Meanwhile, the protein purified with the Ni-NTA Spin Columns was 0.35 mg/mL, its hemolysis activity was 1463 HU/mg, and hemolysin yield was 17.5%, respectively. The results showed that there is no significant difference in the quality, hemolysis activity and yield of the recombinant proteins purified with Ni-NTA spin columns and GFC.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Compostos Organometálicos / Staphylococcus aureus / Proteínas Recombinantes / Cromatografia em Gel / Escherichia coli / Genética / Proteínas Hemolisinas / Metabolismo / Métodos / Ácido Nitrilotriacético Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Compostos Organometálicos / Staphylococcus aureus / Proteínas Recombinantes / Cromatografia em Gel / Escherichia coli / Genética / Proteínas Hemolisinas / Metabolismo / Métodos / Ácido Nitrilotriacético Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo