Construction of engineered Escherichia coli for aerobic succinate production / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2081-2085, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-302870
ABSTRACT
Based on carbon metabolic pathway analysis of Escherichia coli MG1655, an aerobic succinate fermentation platform was constructed by knocking out five genes (ptsG, poxB, pta, iclR and sdhA), which was named E. coli QZ1111. Flask cultivation results showed that E. coli QZ1111 could accumulate succinate with a concentration of 26.4 g/L under aerobic conditions. The byproduct acetate was only 2.3 g/L. The production ratio of succinate and acetate reached 11.51.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Engenharia de Proteínas
/
Regulação Bacteriana da Expressão Gênica
/
Ácido Succínico
/
Aerobiose
/
Escherichia coli
/
Técnicas de Inativação de Genes
/
Fermentação
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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