Cloning and function analysis of the rice small GTP-binding protein gene OsPra2 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2027-2033, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-302877
ABSTRACT
Gene expression in rice roots under nutritional stress was studied using micro array techniques. The results showed that when re-supplied with sufficient amounts of nutrition after nutrition stress, the expression of OsPra2 (a small G protein which is homologous with Pea Pra2 protein) decreased in the plants root tissue. The cDNA sequence of the OsPra2 gene and its promoter, which is about 1 kb upstream of the translation origin point, was obtained using RT-PCR and PCR approaches. The OsPra2 protein contains four conserved GTP/GDP binding domains and specific domain of Rab small G protein family. The expression of OsPra2 and GST fusion protein in onion epidermal cells showed that OsPra2 protein was localized in the membrane and nucleus of the cell. The fusion expression of OsPra2 promoter and GUS reporter gene in transgenic rice suggested that the OsPra2 promoter allowed GUS expression in coleoptiles and roots. Compared with wild type rice, OsPra2 over expressed transgenic rice showed an obvious dwarf phenotype which resembles the BR deficient rice.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Plantas
/
Oryza
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Sequência de Aminoácidos
/
Plantas Geneticamente Modificadas
/
Clonagem Molecular
/
Homologia de Sequência de Aminoácidos
/
Genes de Plantas
/
Regulação da Expressão Gênica de Plantas
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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