Role of heterogeneous nuclear ribonucleoprotein A2/B1 protein in the pathogenesis of non-small cell lung cancer / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 110-114, 2011.
Article
em Zh
| WPRIM
| ID: wpr-303356
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) in non-small cell lung cancer (NSCLC), and the interaction between hnRNP A2/B1 protein and mRNA of DNA repair enzymes O(6)-methylguanine DNA-methyltransferase (MGMT), 8-oxoguanine DNA glycosylase (OGG1), redox factor 1(Ref-1), DNA-dependent protein kinase (including DNA-PKcs and ku).</p><p><b>METHODS</b>The expression and distribution of hnRNP A2/B1 were detected by immunohistochemistry and Western blot on 50 NSCLC samples from patients who underwent resection in Zhongshan Hospital. The hnRNP A2/B1 mRNA expression was tested by real-time PCR. Co-immunoprecipitation (co-IP) combined RT-PCR was used to investigate whether hnRNP A2/B1 could be bound with the mRNA of the above mentioned 5 DNA repair enzymes in human lung cancer cell line (HTB-182). Then immunohistochemistry and real-time PCR were used to detect the expression of MGMT in the same group of patients.</p><p><b>RESULTS</b>HnRNP A2/B1 protein and mRNA expressions were increased in the NSCLC tissues than that in the corresponding normal lung tissues. HnRNP A2/B1 was expressed predominantly in the nuclei of tumor cells. The positive rate and immunohistochemistry score of hnRNP A2/B1 in tumor tissue were significantly higher than that in normal tissue (P < 0.01). In stage III-IV NSCLC, hnRNP A2/B1 expression was higher than that in stage I-II. There was no significant differences of hnRNP A2/B1 expression among patients of different age, sex, histological type, and smoking history. The results of co-IP combined RT-PCR suggested that hnRNP A2/B1 is bound with MGMT mRNA, and MGMT expression is decreased in tumor tissue of NSCLC.</p><p><b>CONCLUSIONS</b>The results of this study show that hnRNP A2/B1 protein and mRNA are highly expressed in NSCLC, and hnRNP A2/B1 is bound with MGMT mRNA, which indicate that it might be one of the mechanisms of hnRNP A2/B1 participating in the pathogenesis of NSCLC.</p>
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Índice:
WPRIM
Assunto principal:
RNA Mensageiro
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Imuno-Histoquímica
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Química
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Western Blotting
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Carcinoma Pulmonar de Células não Pequenas
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Ribonucleoproteínas Nucleares Heterogêneas Grupo A-B
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Imunoprecipitação
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Proteína Quinase Ativada por DNA
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Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2011
Tipo de documento:
Article