Construction and confirmation of a recombinant adenovirus vector of survivin / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 13-17, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-303377
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer.</p><p><b>METHODS</b>The survivin gene was cloned by PCR. After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector. The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus.</p><p><b>RESULTS</b>The sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed.</p><p><b>CONCLUSIONS</b>A survivin recombinant adenovirus has been successfully constructed.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Proteínas Recombinantes de Fusão
/
Transfecção
/
Adenoviridae
/
Reação em Cadeia da Polimerase
/
Proteínas Inibidoras de Apoptose
/
Células HEK293
/
Vetores Genéticos
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2011
Tipo de documento:
Artigo
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