Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: comparison of three staining methods
Asian Pacific Journal of Tropical Biomedicine
;
(12): 61-65, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-303624
ABSTRACT
<p><b>OBJECTIVE</b>To compare the efficacy of three different tissue stains, namely haematoxylin and eosin (H&E), periodic-acid Schiff (PAS) and immunohistochemical (IHC) stains for detection of Entamoeba histolytica (E. histolytica) trophozoites in abscessed liver tissues of hamster.</p><p><b>METHODS</b>Amoebic liver abscess was experimentally induced in a hamster by injecting 1 × 10(6) of axenically cultured virulent E. histolytica trophozoites (HM1-IMSS strain) into the portal vein. After a week post-inoculation, the hamster was sacrificed and the liver tissue sections were stained with H&E, PAS and IHC stains to detect the amoebic trophozoite.</p><p><b>RESULTS</b>The three stains revealed tissue necrosis and amoebic trophozoites, but with varying clarity. H&E and PAS stained the trophozoites pink and magenta, respectively, however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology. On the other hand, IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.</p><p><b>CONCLUSIONS</b>It can be concluded that out of the three stains, IHC is the best for identification of E. histolytica trophozoites in tissue sections.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Patologia
/
Coloração e Rotulagem
/
Imuno-Histoquímica
/
Mesocricetus
/
Biologia Celular
/
Diagnóstico
/
Modelos Animais de Doenças
/
Entamoeba histolytica
/
Trofozoítos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS