Your browser doesn't support javascript.
loading
Study on the proliferation of epidermal cells of wound edge in deep partial thickness scald injury in rat / 中华烧伤杂志
Chinese Journal of Burns ; (6): 128-131, 2005.
Artigo em Chinês | WPRIM | ID: wpr-303676
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the rule and possible mechanism of epidermal proliferation in wound edge of deep partial thickness scald injury in rat.</p><p><b>METHODS</b>Twenty-four Sprague-Dawley rats inflicted with deep partial thickness scald were randomized into pre-scalding, 3 post-scalding day (PSD), 7PSD and 14PSD groups, with 6 rats in each group. Skin specimens from the wound edge were harvested for the observation of the histological characteristics of the epidermis. Cell cycles of epidermal cells were analyzed with flow cytometry. The expressions of cyclin D1, cyclin B1, cdk4 and the histone H1 kinase activity of MPF in epidermal cells were determined by Western blotting.</p><p><b>RESULTS</b>Augmentation of nuclei and nucleoli was found in the epidermal cells from the wound edge in 3PSD group, while increased number of epidermal cells with obviously augmented nuclei and nucleoli were found in 14PSD group. The percentage of the cells in S phase increased in 14 PSD group. The percentage of epidermal cells in G2/M phase began to increase in 3PSD group, and that in 7PSD (4.5 +/- 0.6) and 14PSD (5.4 +/- 1.0) groups were obviously higher than that in pre-scalding group (2.9 +/- 1.1, P < 0.05). The expression of cyclin D1 increased significantly in 3PSD group. The expression of cdk4 decreased in 3PSD group, but began to increase in 14PSD group. There was no difference in the expression of cyclin B1 among groups. The MPF activity was significantly increased in 14PSD group.</p><p><b>CONCLUSION</b>There was enhanced DNA synthesis and mitosis in epidermal cells of rats with deep partial scald during early post-scald stage, and active proliferation of epidermal cells was observed on 14PSD. The expression of cyclinD1/cdk4 complex and the activity of MPF increased since 14PSD, indicating that there was a special regulative pattern during wound healing.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Cicatrização / Queimaduras / Ciclo Celular / Ratos Sprague-Dawley / Lesões dos Tecidos Moles / Biologia Celular / Proliferação de Células / Modelos Animais de Doenças / Células Epiteliais Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Burns Ano de publicação: 2005 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Cicatrização / Queimaduras / Ciclo Celular / Ratos Sprague-Dawley / Lesões dos Tecidos Moles / Biologia Celular / Proliferação de Células / Modelos Animais de Doenças / Células Epiteliais Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Burns Ano de publicação: 2005 Tipo de documento: Artigo