Protective effects of bactericidal/permeability increasing protein simulated peptide on murine acute lung injury induced by lipopolysaccharide / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 100-103, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-303685
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of bactericidal/permeability increasing protein simulated peptide (bactericidal neutralizing endotoxin protein, BNEP) on murine acute lung injury (ALI) induced by lipopolysaccharide (LPS).</p><p><b>METHODS</b>A murine model of ALI was reproduced by lipopolysaccharide via intranasal instillation. The Balb/c mice were randomly divided into control (n = 20, with nasal instillation of isotonic saline), LPS instillation (n = 20, with nasal instillation of isotonic saline and LPS) and BNEP treatment (n = 20, with nasal instillation of isotonic saline plus LPS and BNEP) groups. The ratio of lung wet weight to dry weight, the permeability of pulmonary capillary vessels and the histopathology of pulmonary tissue were determined in all groups. The change in the expression of Toll-like receptor 2 and 4 (TLR2/4) in the pulmonary tissue was detected by immunohistochemistry.</p><p><b>RESULTS</b>Compared with LPS instillation group, the ratio of lung wet weight to dry weight and the permeability of pulmonary capillary vessel was decreased significantly in the BNEP group, and the inflammatory infiltration in the pulmonary tissue induced by neutrophil influx was alleviated markedly with BNEP treatment. The expression of TLR2 and TLR4 in pulmonary vascular endothelial cells, macrophages and alveolar type II epithelial cells in BNEP group were lower than those in LPS group (TLR2 128 +/- 10 vs 214 +/- 12, P < 0.01).</p><p><b>CONCLUSION</b>BNEP, as a simulated peptide of BPI, exerted a remarkable protective effect on ALI induced by LPS.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Permeabilidade Capilar
/
Proteínas Sanguíneas
/
Barreira Alveolocapilar
/
Lipopolissacarídeos
/
Peptídeos Catiônicos Antimicrobianos
/
Modelos Animais de Doenças
/
Lesão Pulmonar Aguda
/
Pulmão
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Burns
Ano de publicação:
2005
Tipo de documento:
Artigo
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