Effect of Single and Double Administration of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Following Focal Cerebral Ischemia in Rats
Experimental Neurobiology
;
: 55-65, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-30376
ABSTRACT
Stem cell therapies are administered during the acute phase of stroke to preserve the penumbral tissues from ischemic injury. However, the effect of repeated cell therapy during the acute phase remains unclear. In this study, we investigated and compared the functional outcome of single (two days post-injury) and repeated (two and nine days post-injury) treatment with human umbilical cord derived mesenchymal stem cells (hUCB-MSCs) after middle cerebral artery occlusion (MCAO). The rotarod and limb placement tests were utilized to investigate functional outcomes, while infarct volume and tissue damage were measured by immunofluorescent staining for neovascularization, neurogenesis, apoptosis, and inflammation in the penumbral zones. We observed notable motor dysfunction and a significant decrease in infarcted brain volume, as well as increases in neurons and vessels in both single and repeated hUCB-MSC treatments compared to the control group. Interestingly, repeated administration of hUCB-MSCs was not found to elicit additional or synergistic improvements over monotherapy. This study suggests that a clearer understanding of the therapeutic window after stroke will facilitate the development of more efficient treatment protocols in the clinical application of stem cell therapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Cordão Umbilical
/
Encéfalo
/
Isquemia Encefálica
/
Protocolos Clínicos
/
Apoptose
/
Acidente Vascular Cerebral
/
Infarto da Artéria Cerebral Média
/
Extremidades
/
Neurogênese
Tipo de estudo:
Guia de Prática Clínica
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Experimental Neurobiology
Ano de publicação:
2017
Tipo de documento:
Artigo
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