Agrobacterium-mediated genetic transformation of secondary somatic embryos in alfalfa / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 203-213, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-304500
ABSTRACT
We describe a genetic transformation method of secondary somatic embryogenesis in alfalfa through cotyledon-stage somatic embryos of alfalfa infected by Agrobacterium strain GV3101. The Agrobacterium strain GV3101 contained binary vector pCAMBIA2301 that had gus gene as reporter and npt II gene as selectable marker. The infected primary embryos were induced through series of medium under 75 mg/L kanamycin selection. We obtained the transgenic alfalfa plants. Then, GUS expression in different tissue of transgenic alfalfa was tested by GUS histochemical analysis. Further, the stable integration and transformation efficiency were tested by polymerase chain reaction and Southern blotting hybridization. The result showed that GUS expression was different in different organs of transgenic alfalfa; the copy number of integrated npt II gene was from 1 to 4; the transformation efficiency via primary somatic embryogenesis was 65.82%.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Transformação Genética
/
Embriologia
/
Plantas Geneticamente Modificadas
/
Técnicas de Cultura de Tecidos
/
Medicago sativa
/
Técnicas de Embriogênese Somática de Plantas
/
Agrobacterium
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo
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