Progress in the sequence and structure properties, thermostability mechanism and molecular modification of xylose isomerase: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1690-1701, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-304531
ABSTRACT
With the development of low-carbon economy and renewable resource, fermentation of the pentose sugar xylose to produce ethanol becomes a very hot topic. The recombinant Saccharomyces cerevisiae can be constructed by expressing heterologous xylose isomerase (XI). Because Thermus thermophilus XI (TthXI) does not need cofactor, it has been developed for establishing the utilization pathway of xylose in S. cerevisiae. In this article, we reviewed the progress on xylose isomerase. We first introduced the primary properties, sequence and structure characters of xylose isomerase, and discussed its thermostability. The molecular modification of xylose isomerase, including of substrate specificity and thermostability were discussed in detail. Meanwhile, combined with our own research, we also discussed how to improve the xylose isomerase activity at room temperature. Finally, we suggested perspectives of xylose isomerase.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Saccharomyces cerevisiae
/
Especificidade por Substrato
/
Estabilidade Enzimática
/
Proteínas Recombinantes
/
Catálise
/
Química
/
Aldose-Cetose Isomerases
/
Genética
/
Temperatura Alta
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo
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