Expression of a protein elicitor pebC1 from Botrytis cinerea in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1631-1636, 2011.
Article
em Zh
| WPRIM
| ID: wpr-304537
Biblioteca responsável:
WPRO
ABSTRACT
In order to express PebC1 in Pichia pastoris, the pebC1 sequence was amplified from genome Botrytis cinerea BC-4-2-2-1 by PCR and subcloned into the Pichua pastoris expression vector pPIC9K to generate pPIC9K-pebC1. The recombinant plasmid was linearized by Bgl II and transformed into Pichia pastoris GS115 by electroporation. Recombinant Pichia pastoris GS115/pPIC9K-pebC1 was screened by MD and G418-YPD plates and further confirmed by PCR. The protein expression was induced by methanol and analyzed by SDS-PAGE. SDS-PAGE analysis showed a special band about 39 kDa and western blotting indicated a good antigenicity of the expressed protein. Bioassay results showed that the recombinant protein PebC1 can induce resistance to gray mould disease of cucumber and Arabidopsi thaliana.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Pichia
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Doenças das Plantas
/
Proteínas Recombinantes
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Proteínas Fúngicas
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Química
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Botrytis
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Genética
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Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Article