Establishment of RAW264.7 cell line stably expressing Mycobacterium tuberculosis protein ESAT-6 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1390-1396, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-304564
ABSTRACT
For studying the effects of Mycobacterium tuberculosis secretory protein ESAT-6 on the related functions of macrophages, RAW264.7 cells were transfected with pEGFP-C1-ESAT-6 and pEGFP-C1 by liposome respectively. After screening with a high level of G418, the macrophage cell lines that stably expressed EGFP-ESAT-6 fusion protein or EGFP were established. The gene and protein expression levels were further analyzed by RT-PCR, fluorescence microscopy and Western blotting. The results indicated that the EGFP-ESAT6 fusion gene was integrated into the chromosome and the protein could be stably expressed in the selected macrophage cell line. These results gave us a tool for the future study in the mechanisms of ESAT-6 protein in modulating the macrophage cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Bactérias
/
Proteínas Recombinantes de Fusão
/
Transfecção
/
Linhagem Celular
/
Biologia Celular
/
Proteínas de Fluorescência Verde
/
Genética
/
Macrófagos
/
Metabolismo
/
Antígenos de Bactérias
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS