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Effect of Xinmailong on hypoxia-inducible factor-1alpha expression in neonatal rats with asphyxia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 683-686, 2009.
Artigo em Chinês | WPRIM | ID: wpr-304616
ABSTRACT
<p><b>OBJECTIVE</b>Xinmailong, a compound extracted from Periplaneta americana, is used for the treatment of cardiovascular diseases. This study investigated the effects of Xinmailong on myocardial hypoxia-inducible factor-1alpha (HIF-1alpha) and plasma endothelin-1(ET-1) levels in neonatal rats with asphyxia and explored the protection mechanism of Xinmailong in hypoxia-ischemic myocardial injury.</p><p><b>METHODS</b>Seven-day-old Sprague-Dawley rats were randomly divided into three groups (n=30 each) sham-operated, asphyxia, Xinmailong-treated asphyxia. Each group was randomly subdivided into three groups according to the observed time points 6 hrs, 24 hrs and 72 hrs. Xinmailong (5 mg/kg) was intraperitoneally injected to the rats in the Xinmailong-treated group five minutes before asphyxia. Myocardial HIF-1alpha expression, and plasma ET-1 and creatine kinase (CK) levels were measured. The histopathologic changes of the myocardium were observed by hematoxylin-eosin staining.</p><p><b>RESULTS</b>Four rats died in the asphyxia group while only one died in the Xinmailong-treated group during the experiment. The plasma ET-1 and CK levels as well as myocardial HIF-1alpha expression increased at 6 hrs, reached a peak at 24 hrs, and declined at 72 hrs after asphyxia in the asphyxia group, being higher than that in the sham-operated group (P<0.01). Myocardial ischemia was observed in the three time points, and cell necrosis occurred at 24 hrs after asphyxia in the asphyxia group. Myocardial HIF-1alpha expression was positively correlated with plasma ET-1 levels (r=0.876, P<0.01). In the Xinmailong-treated group, plasma levels of CK and ET-1 as well as myocardial HIF-1alpha expression were significantly lower than those in the asphyxia group (P<0.01). Myocardial ischemia was alleviated and no cell necrosis was found in the Xinmailong-treated group.</p><p><b>CONCLUSIONS</b>Asphyxia leads to increase in myocardial HIF-1alpha expression and plasma levels of ET-1 and CK. Xinmailong can reduce the myocardial expression of HIF-1alpha and decrease plasma ET-1 levels, thus alleviating hypoxia-ischemic myocardial injury.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Periplaneta / Asfixia / Sangue / Imuno-Histoquímica / Química / Ratos Sprague-Dawley / Isquemia Miocárdica / Endotelina-1 / Creatina Quinase Limite: Animais Idioma: Chinês Revista: Chinese Journal of Contemporary Pediatrics Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Periplaneta / Asfixia / Sangue / Imuno-Histoquímica / Química / Ratos Sprague-Dawley / Isquemia Miocárdica / Endotelina-1 / Creatina Quinase Limite: Animais Idioma: Chinês Revista: Chinese Journal of Contemporary Pediatrics Ano de publicação: 2009 Tipo de documento: Artigo