Correlation of oxidative stress with sperm DNA integrity and semen parameters in infertile men with varicocele / 中华男科学杂志
National Journal of Andrology
; (12): 530-533, 2016.
Article
em Zh
| WPRIM
| ID: wpr-304705
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WPRO
ABSTRACT
<p><b>Objective</b>To investigate the relationship of oxidative stress with DNA integrity and semen parameters in infertile men with varicocele (VC).</p><p><b>METHODS</b>This prospective study included 98 infertile males with VC. According to the levels of reactive oxygen species (ROS) in the semen, we divided the patients into a high ROS group (n=44) and a low ROS group (n=54), determined the sperm DNA fragmentation index (DFI), motility and morphology, and analyzed their correlation with ROS in the two groups of patients.</p><p><b>RESULTS</b>Compared with the patients of the low ROS group, those of the high ROS group showed a significantly higher DFI (27.38±8.10 vs 34.49±6.05, P=0.039) and a higher concentration of seminal leukocytes ([0.65±0.15]×10⁶/ml vs [0.86±0.41]×10⁶/ml, P=0.022), but lower sperm motility ([36.16±22.83]% vs [18.22±25.21]%, P=0.017), percentage of progressively motile sperm ([23.34±11.53]% vs [16.34±9.22]%, P=0.041), sperm curvilinear velocity ([27.03±6.21] vs [20.62±4.38] μm/s, P=0.013), and sperm linearity ([29.75±8.24]% vs [18.30±7.93]%, P=0.024). Spearman correlation analysis indicated that the ROS level was correlated positively with the concentration of seminal leukocytes (r=0.41, P<0.01) and DFI (r=0.21, P=0.006), but negatively with sperm curvilinear velocity (r=-0.24, P=0.017), linearity (r=-0.24, P=0.021), motility (r=-0.31, P=0.002), and the percentage of progressively motile sperm (r=-0.41, P=0.012). Additionally, the sperm DFI manifested a significant negative correlation with sperm motility (r=-0.29, P<0.01) and the percentage of progressively motile sperm (r=-0.34, P<0.01).</p><p><b>CONCLUSIONS</b>The level of seminal ROS is positively correlated with the sperm DFI in infertile men with varicocele, and both the ROS level and DNA integrity are associated with semen parameters.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Sêmen
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Motilidade dos Espermatozoides
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Espermatozoides
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Varicocele
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Estudos Prospectivos
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Espécies Reativas de Oxigênio
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Estresse Oxidativo
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Fragmentação do DNA
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Infertilidade Masculina
Tipo de estudo:
Observational_studies
Limite:
Humans
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Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2016
Tipo de documento:
Article