High-throughout detection and analysis of drug-resistance gene mutation for 2465 cases with chronic hepatitis B in Shenzhen / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the character of drug-resistance gene mutation for patients with chronic hepatitis B in Shenzhen.</p><p><b>METHODS</b>2465 clinical cases with chronic hepatitis B were analyzed for gene mutation with MALDI-TOF-MS in order to know about the epidemiology of HBV drug resistance and its clinical significance.</p><p><b>RESULTS</b>763 cases were detected mutation among the 2465 cases. The frequency of Lamivudine related mutation was the highest (42.96%), especially on rtL180M (14. 72%), rtL204I (18. 50%), rtL204V (9. 74%). The frequency of Adefovir related mutation was about 8. 19% , among of which rtN236T was 4. 15%. The frequency of Entecavir related mutation was about 0. 49%. Among all samples, rtS202I mutation couldn't be detected. The existence of drug resistance could be detected earlier with MALDI-TOF-MS from the results of dynamic follow-up.</p><p><b>CONCLUSION</b>In Shenzhen, the main HBV mutation was associated with lamivudine and adefovir,and with lower frequency of mutation for entecavir,so the optimized treatment for HBV was entecavir. It could detect the existence of drug resistance effectively with MALDI-TOF-MS and guide clinical treatment.</p>