Hepatitis A virus mimotope mapping by phage display peptide library / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 456-459, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-305010
ABSTRACT
<p><b>OBJECTIVE</b>A 12 mer phage display peptide library was used to identify hepatitis A virus mimotopes of antigenic determinants, to provide the feasibility of virus epitope mapping by using this approach.</p><p><b>METHODS</b>Using purified anti-hepatitis A virus monoclonal antibody as affinity selective molecule, phage display peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>10 ELISA positive clones were chosen for DNA sequencing, and the displayed peptide sequences were deduced. 9 of them showed identical nucleotide sequence, and similarity in their amino acid sequence with VP1 of HAV HM175 was found, but no sequence homology was found between the other phage clone and the capsid proteins of HAV. Those peptides may behave as mimotopes of HAV.</p><p><b>CONCLUSION</b>The mimotope of HAV was selected by using phage display peptide library screening. The results provide the potential of this method to search for the mimotopes of the virus.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Dados de Sequência Molecular
/
Química
/
Sequência de Aminoácidos
/
Mapeamento de Epitopos
/
Biblioteca de Peptídeos
/
Vírus da Hepatite A
/
Alergia e Imunologia
/
Genética
/
Hepatite A
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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