Study on the correlation between membrane protein Flotillin-1 and PrPc endocytosis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 435-438, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-305016
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPc.</p><p><b>METHODS</b>The expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPc in Cells which were treated with copper ions was observed using immunoprecipitation method.</p><p><b>RESULTS</b>(1) Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; (2) Only in the appearance of copper ions, the protein complexes of PrPc and Flotillin-1 can be detected with the method of IP, which were related to copper ions concentration and processing time.</p><p><b>CONCLUSION</b>The membrane-associated protein Flotillin-1 has the relationship with the endocytosis of PrPc.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Linhagem Celular
/
Membrana Celular
/
Proteínas PrPC
/
Transporte Proteico
/
Endocitose
/
Genética
/
Proteínas de Membrana
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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