Mutant D178N prion protein converts spontaneously in RT-QuIC assay / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 370-373, 2012.
Article
em Zh
| WPRIM
| ID: wpr-305032
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the conversion of mutant D178N prion protein in RT-QuIC assay.</p><p><b>METHODS</b>The D178N mutant prion PRNP was generated by the method of single site mutation. The mutant PRNP gene was inserted into plasmids of pET24. The full and N-truncated recombinant human prion proteins were expressed and purified. The fibril formations of these proteins were real-time monitored by the method of RT-QuIC. The ability to resist proteinase K (PK) of these fibrils was analyzed.</p><p><b>RESULTS</b>We succeed to construct human PrP-D178N plamids. The N-truncated human prion protein with D178N (PrP90-231-D178N) can convert spontaneously in RT-QuIC, while full length of human prion D178N protein (PrP23-231-D178N) fails to convert spontaneously. The spontaneously generated fibril has been domenstrated it is partily PK-resistant.</p><p><b>CONCLUSION</b>The N-terminal of prion protein (23-90) plays an important role for the D178N mutant protein spontaneously conversion, which provide the clues for study the pathogenesis of genetic CJD.</p>
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Índice:
WPRIM
Assunto principal:
Príons
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Síndrome de Creutzfeldt-Jakob
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Técnicas de Amplificação de Ácido Nucleico
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Proteínas Mutantes
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Genética
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Métodos
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2012
Tipo de documento:
Article