Primary establishment of an alphaLISA assay for detection of HAV IgM / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an AlphaLISA method for detection of antibody of Hepatitis A virus.</p><p><b>METHODS</b>After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.</p><p><b>RESULTS</b>The sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%.</p><p><b>CONCLUSION</b>A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.</p>