Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 29-31, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-305115
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-gamma and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP.</p><p><b>METHODS</b>Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-gamma and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls.</p><p><b>RESULTS</b>Blood T-bet mRNA expression and IFN-gamma levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (p<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (p<0.01).</p><p><b>CONCLUSIONS</b>The high expression of T-bet and IFN-gamma and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
RNA Mensageiro
/
Interleucina-4
/
Interferon gama
/
Púrpura Trombocitopênica Idiopática
/
Células Th1
/
Proteínas com Domínio T
/
Alergia e Imunologia
/
Fator de Transcrição GATA3
/
Genética
Limite:
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
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