Expression of SARS spike gene in Shizomycete pombe / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 638-641, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-305189
ABSTRACT
The viral spike protein is the main surface antigen of the coronavirus, and it could be useful in the research of clinical diagnosis, SARS vaccine and the structure biology.According to the analysis of the main antigen of the SARS spike protein, 5 fragments of the whole spike gene were cloned, and ligated to the vector pNMT1. Through electroporation transformantion to TCP1, the recombinant S. pombe strains capable of expressing the 5 fragments were constructed. SDS-PAGE or Western blot analysis of the induced expression products demonstrated that the 5 recombinant proteins were expressed in the fission yeast respectively.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Schizosaccharomyces
/
Proteínas Recombinantes
/
Glicoproteínas de Membrana
/
Proteínas do Envelope Viral
/
Clonagem Molecular
/
Eletroporação
/
Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
/
Glicoproteína da Espícula de Coronavírus
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
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